{"id":1681,"date":"2025-02-03T02:46:03","date_gmt":"2025-02-03T02:46:03","guid":{"rendered":"https:\/\/basiclifetw.com\/?page_id=1681"},"modified":"2025-06-03T00:37:26","modified_gmt":"2025-06-03T00:37:26","slug":"100-10000-bp-dna-marker","status":"publish","type":"page","link":"https:\/\/basiclifetw.com\/zh_tw\/%e7%94%a2%e5%93%81\/%e8%a9%a6%e5%8a%91\/100-10000-bp-dna-marker\/","title":{"rendered":"100\u201310,000 bp DNA Marker"},"content":{"rendered":"Home<\/a><\/span><\/span>\n\n\n\n
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\"\"<\/figure><\/div>
\"\"<\/figure><\/div><\/div><\/div><\/div>\n\n\n\n
\n

100\u201310000 bp DNA Marker<\/em><\/h2>\n\n\n\n

100 bp \u2013 10,000 bp DNA Marker<\/em><\/em><\/p>\n\n\n\n

\u8aaa\u660e :<\/strong><\/p>\n\n\n\n

\u9810\u5148\u7528\u6700\u654f\u611f\u7684\u87a2\u5149\u67d3\u6599\u4e4b\u4e00\u67d3\u8272\u3002\u6b64\u68af\u5ea6\u70ba\u5373\u7528\u578b\u5f62\u5f0f\uff0c\u542b\u6709\u87a2\u5149 DNA \u67d3\u8272\u5291\u548c\u793a\u8e64\u67d3\u6599\uff0c\u4e26\u4e14\u91dd\u5c0d\u76f4\u63a5\u52a0\u8f09\u5230\u672a\u67d3\u8272\u7684\u74ca\u8102\u7cd6\u51dd\u81a0\u4e0a\u9032\u884c\u4e86\u512a\u5316\u3002\u6b64\u5916\uff0c\u5b83\u5728\u65e5\u5e38\u64cd\u4f5c\u904e\u7a0b\u4e2d\u63d0\u4f9b\u4e86\u6700\u5927\u7684\u4fbf\u5229\uff0c\u907f\u514d\u4e86\u5e38\u7528\u7684\u51dd\u81a0\u67d3\u8272\u7a0b\u5e8f\uff0c\u5982\u6eb4\u5316\u4e59\u9320\u6216SYBR\u00ae Green I\u3002 \uff0c\u4f46\u4e0d\u80fd\u52a0\u5230\u68af\u5b50\u4e0a\u3002<\/p>\n\n\n\n

BL1097 \u5305\u542b 100 \u81f3 10,000 \u500b\u9e7c\u57fa\u5c0d\u7684\u7247\u6bb5\u3002 1K \u548c 3K \u6ce2\u6bb5\u7684\u5f37\u5ea6\u589e\u52a0\uff0c\u53ef\u4f5c\u70ba\u53c3\u8003\u9ede\u3002\u63d0\u4f9b\u4e86\u6bcf\u500b\u5e36\u4e2d\u7684 DNA \u8fd1\u4f3c\u8cea\u91cf\uff08\u6bcf\u6b21\u52a0\u8f09 0.5 \u03bcg\uff09\uff0c\u7528\u65bc\u8fd1\u4f3c\u4f30\u7b97\u985e\u4f3c\u5927\u5c0f\u7684\u5f37\u5ea6\u76f8\u7576\u7684\u6a23\u672c\u4e2d\u7684 DNA \u8cea\u91cf\u3002<\/p>\n\n\n\n

Source : <\/strong>PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.<\/p>\n\n\n\n

Reaction Setup: <\/strong>6 \u03bcl \/ well, containing bromophenol blue & xylene cyanol FF as tracking dyes.<\/p>\n\n\n\n

Range: <\/strong>100 – 10,000 bp<\/p>\n\n\n\n

Number of bands: <\/strong>13<\/p>\n\n\n\n

\u5132\u5b58 \uff1a<\/strong><\/p>\n\n\n\n

\u5728 25\u00b0C \u4e0b\u53ef\u7a69\u5b9a\u4fdd\u5b58\u9577\u9054 6 \u500b\u6708\u3002
\u5728 4\u00b0C \u4e0b\u53ef\u7a69\u5b9a\u4fdd\u5b58\u9577\u9054 12 \u500b\u6708\u3002
\u5728-20\u00b0C \u4e0b\u53ef\u7a69\u5b9a\u4fdd\u5b58\u9577\u9054 24 \u500b\u6708\u3002<\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/em><\/strong><\/th>\u7522\u54c1\u6558\u8ff0<\/em><\/strong><\/th>\u5305\u88dd<\/em><\/strong><\/th><\/tr><\/thead>
BL1097<\/td>100 bp \u2013 10,000 bp DNA Marker<\/em><\/em><\/em><\/td>600\u03bcl\/bot<\/td><\/tr><\/tbody>
<\/td><\/td><\/td><\/tr><\/tfoot><\/table>
*\u7522\u54c1\u7684\u5be6\u969b\u984f\u8272\u53ef\u80fd\u56e0\u62cd\u651d\u5149\u7dda\u3001\u986f\u793a\u8a2d\u5099\u6216\u5176\u4ed6\u689d\u4ef6\u5f71\u97ff\u800c\u8207\u5716\u7247\u7565\u6709\u5dee\u7570\uff0c\u8acb\u4ee5\u5be6\u969b\u7684\u5546\u54c1\u70ba\u6e96\u3002<\/figcaption><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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